Analysis Workflow Framing

Standard

Canonical step list vs declared steps by assay (RNA-seq bulk / single-cell, WGS / WES, ChIP-seq / ATAC-seq, methylation, proteomics, metabolomics, 16S / metagenomics, variant-calling). Workflow-level framing only.

3-6 minutes

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Sample / Metadata Readiness

Standard

Sample sheet readiness review — group / batch / date / covariate presence, imbalance, and batch × group confounding detection. Flags missing metadata and underpowered groups.

3-6 minutes

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QC / Pipeline Review

Standard

Assay-specific QC checklist review (FastQC, alignment rate, TSS enrichment, FRiP, coverage, bisulfite conversion, PSM FDR, etc.) vs declared QC and optional measured values. Heuristic thresholds — NOT clinical cutoffs.

3-6 minutes

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Pathway / Result Interpretation

Standard

Interpretation memo over declared enrichment results (FDR filter, small-overlap flag, source diversity). Does NOT re-run enrichment.

3-6 minutes

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Differential Analysis Framing

Standard

Recommended statistical approach + tool by data type (RNA-seq counts / microarray / proteomics / metabolomics / ATAC peaks / methylation / 16S) with sample-size and batch-adjustment flags.

3-6 minutes

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Biological Reproducibility Memo

Standard

Reproducibility practice coverage by share-mode (internal / collaborator / public / clinical): tool versions, reference genome, pipeline versioning, raw-data deposit, IRB, clinical SOP, QC archive. Advisory — does NOT audit claims.

3-6 minutes

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